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KMID : 0861120150190030141
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Korean Journal of Oriental Preventive Medicine
2015 Volume.19 No. 3 p.141 ~ p.154
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Antioxidant and Protective Effects of Palmul-tang on Ultraviolet B (UVB)-induced Damage in Human Keratinocytes
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Kim Tae-Yeon
Park Jong-Phil
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Abstract
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Objective : In this paper, we investigated the anti-oxidative capacities and protective effects of water extract of palmul-tang (PMT) against Ultraviolet B(UVB)-induced oxidative damage in human keratinocytes(HaCaT).
Method : To evaluate the anti-oxidative activities of PMT, we measured scavenging activities on 1,1-diphenyl- 2-picrylhydrazyl(DPPH) radical, hydroxyl radical, hydrogen peroxide, superoxide anion, lipid peroxidation and reducing power of PMT. To give an oxidative stress to HaCaT cells, UVB was irradiated with 40 mJ/㎠ to HaCaT cells. To detect the protective effects of PMT against UVB, we measured cell viability, apoptotic bodies and reactive oxygen species(ROS) generation.
Results : PMT showed the anti-oxidative activities by scavenging DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion, lipid peroxidation. Also PMT showed high reducing values. The UVB-induced oxidative conditions led to the cell apoptosis. However, treatment with PMT reduced oxidative stress conditions, including inhibition of cell apoptosis and expression of ROS.
Conclusion : PMT had anti-oxidative activities and exhibited protective effects against UVB on HaCaT cells. PMT would be useful for the development of cosmetics treating UVB-induced skin aging.
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KEYWORD
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Palmul-tang (PMT), Ultraviolet B(UVB), Human keratinocytes(HaCaT), Anti-oxidative activity, Apoptosis, Reactive oxygen species(ROS)
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